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对由PCR扩增的血管内皮细胞生长因子（VEGF165）cDNA编码N－24位氨基酸错误配对的密码子ACG（应为AGC丝氨酸）进行校正。方法：用一个化学合成的寡聚脱氧核苷酸片断作为诱变引物对VEGF165）cDNA编码N-24位氨基酸错误配对的密码子进行体外定点突变，转化子经打点杂交及温度梯度洗膜，筛选出突变子，再DNA序列分析。结果：VEGF165cDNA编码N-24位氨基酸错误配对的密码子变为AGC（丝氨酸）。结论：该突变方法可靠，省时，实用。
The codon ACG (which should be AGC serine) mismatched with the cDNA encoding Vascular Endothelial Growth Factor (VEGF165) amplified by PCR at amino acid position N-24 was corrected. Methods: A chemically synthesized oligodeoxynucleotide fragment was used as a mutagenic primer to mutate the codon mismatched with N-24 amino acid of VEGF165 cDNA in vitro. The transformants were screened by dot blot hybridization and temperature gradient Out of mutation, then DNA sequence analysis. RESULTS: The mismatch codon for the amino acid encoding N-24 of VEGF165 cDNA changed to AGC (serine). Conclusion: This mutation method is reliable, time-saving and practical.