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通过建立人工体外游离器官灌注系统对实验性异种移植免疫机制进行探讨，并结合免疫荧光病理及ＥＬＩＳＡ天然抗体测定法与该系统所观察结果作一综合分析，试图客观地评价此系统对异种移植实验研究的价值。本实验共分为五个组，Ａ组为对照组（ｎ＝４），即用猪全血灌注猪肾脏；异种基因牛血灌注组为Ｂ组（ｎ＝４）；Ｃ组为羊血灌注组（ｎ＝２）；Ｄ组和Ｅ组分别为猴血（ｎ＝４）和人血组（ｎ＝４）。Ａ组中，灌注持续２００ｍｉｎ均保持较低的肾血管阻力；Ｂ组在１５５ｍｉｎ内仍保持较低的血管阻力；Ｄ组排斥发生非常迅速，仅４ｍｉｎ；Ｅ组在５５ｍｉｎ时血管阻力明显增加；而Ｃ组在第７０ｍｉｎ时出现排斥反应。应用免疫荧光技术，Ｄ、Ｅ组均可见到ＩｇＭ抗体沉积在肾小球间质内，Ｂ组见到较弱的ＩｇＭ沉积，Ｃ组未得到好的结果。本实验中未见到ＩｇＧ在超急排斥中起任何作用。ＥＬＩＳＡ法测定对照组ＩｇＭ抗体水平变化不大。Ｄ组ＩｇＭ吸收率最高（３０．３％），Ｅ组为２２．６％，Ｂ组吸收率最低（１１．２％），Ｃ组为１４．８％。灌注前后抗体ＩｇＭ吸收率变化顺序为Ｄ、Ｅ、Ｃ、Ｂ组，与各实验组超急排斥反应的发生时间顺序一致。结论：本人工体外游离器官灌注系统是一种可靠的研究异种移植免?
Through the establishment of artificial extracorporeal organ perfusion system to explore the mechanism of experimental xenograft immunity, combined with immunofluorescence pathology and ELISA natural antibody assay and the results of the system for a comprehensive analysis, an objective evaluation of the system of xenograft experiments The value of research. The experiment was divided into five groups, A group as control group (n = 4), that is, pig porcine kidney perfusion; heterologous gene cow blood perfusion group B (n = 4); C group sheep blood perfusion (N = 2). Groups D and E were monkey blood (n = 4) and human blood group (n = 4), respectively. In group A, renal vascular resistance remained low for 200 min after perfusion; group B maintained low vascular resistance within 155 min; rejection in group D occurred very rapidly for only 4 min; group V increased vascular resistance at 55 min; C group rejection occurred at 70min. Application of immunofluorescence technology, D, E group can be seen IgM antibody deposition in the glomerular interstitial, B group to see weaker IgM deposition, C group did not get good results. IgG was not found to play any role in hyperacute rejection in this experiment. The level of IgM antibody in the control group changed little by ELISA. The highest IgM absorption rate was found in group D (30.3%), in group E 22.6%, in group B the lowest (11.2%) and group C 14.8%. The order of change of IgM absorption rate before and after perfusion was D, E, C and B groups, which coincided with the occurrence time of hyperacute rejection in each experimental group. Conclusion: The artificial extracorporeal organ perfusion system is a reliable study of xenograft-free?