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苏木精染液的染色力是影响HE染色成功的关键因素之一,而染色力随染色时间和切片数量的增加呈进行性下降。实际工作中技术人员大多依据个人经验来决定苏木精染液是否需要更换,缺乏统一标准,造成批间差异大。本文通过测定苏木精染液使用过程中pH值的变化来确定染液的酸度值,评价苏木精染液的染色力,为实际工作中苏木精染液的更换提供量化参考指标,从而更好地开展HE染色的
The dyeing power of hematoxylin dye solution is one of the key factors that affect the success of HE staining. However, dyeing power decreases progressively with the increase of dyeing time and the number of slices. The actual work of technical staff mostly based on personal experience to decide whether the need to replace the hematoxylin dye, the lack of uniform standards, resulting in large differences between batches. In this paper, by determining the pH value during the use of hematoxylin dye to determine the acidity of the dye liquor, hematoxylin dye staining evaluation of the dyeing force for practical work to replace the hematoxylin dye quantitative reference to provide Better for HE staining