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目的探讨肾母细胞瘤过表达基因(NOV)对肾透明细胞癌细胞基质金属蛋白酶(MMP)表达的影响。方法构建NOV蛋白表达真核细胞重组表达质粒pEGFP-C1-NOV,转染人肾癌细胞株786-O。通过实时定量RT-PCR的方法定量转染pEGFP-C1-NOV细胞(实验组)、转染空载体细胞(空载组)和未转染的786-O细胞(空白组)的基质金属蛋白酶(MMP)亚型(包括MMP-1、MMP-2、MMP-3、MMP-7、MMP-9、MMP-13)的表达水平。方差分析法比较实验组与空载组及空白组各MMP亚型表达水平的差异。结果实验组与空白组和空载组比较,MMP-1、MMP-3和MMP-13表达水平升高(P<0.05),而MMP-2和MMP-7的表达水平下降(P<0.05)。实验组MMP-9的表达水平与空白组和空载组差异无统计学意义(P>0.05)。结论 NOV可调节肾透明细胞癌细胞MMP的表达,NOV对MMP的调节可能是NOV发挥功能的机制之一。
Objective To investigate the effect of overexpression of nephroblastoma (NOV) on the expression of matrix metalloproteinase (MMP) in renal clear cell carcinoma. Methods Recombinant plasmids pEGFP-C1-NOV were constructed and transfected into human renal carcinoma cell line 786-O. Real-time quantitative RT-PCR method was used to quantitatively transfected pEGFP-C1-NOV cells (experimental group), transfected with empty vector cells and untransfected 786-O cells (blank group) matrix metalloproteinase MMP) subtypes (including MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13) The ANOVA method was used to compare the expression of MMP subtypes in experimental group, empty group and blank group. Results Compared with blank group and no-load group, the expression of MMP-1, MMP-3 and MMP-13 in experimental group was significantly increased (P <0.05) . The expression of MMP-9 in the experimental group was not significantly different from that in the blank group and the no-load group (P> 0.05). Conclusion NOV can regulate the expression of MMP in renal clear cell carcinoma, and the regulation of NO by NOV may be one of the mechanisms of NOV.