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研究了从独子藤中提取雷公藤红素的关键提取参数,包括提取时间(10-20 min),提取温度(30-60℃),超声功率(60-90 W)和液料比(10-30 mL/g)对提取效果的影响.采用Box-Behnken Design设计实验方案,通过响应面法,进一步考察了溶剂种类对提取效果的影响.结果表明,在所选的溶剂甲醇、乙醇、乙酸乙酯、正丁醇和水中,乙醇为最佳提取溶剂.采用二阶多项式模型进行数据分析,模型的相关系数R2=0.9928.确定了独子藤中雷公藤红素的较优提取工艺条件为:提取时间20 min,提取温度46℃,提取功率60 W,液料比30 mL/g,按此条件雷公藤红素的提取率可达3.116 mg/g.预测值和实验实测值之间相关性很强.结果表明,应用响应面法优化超声辅助提取独子藤中雷公藤红素的方法高效且可靠.“,”In the present study,we aimed to investigate the effects of key extraction parameters including extraction time (10-20 min),extraction temperature (30-60 ℃),ultrasonic power (60-90 W) and solvent-to-solid (S/S) ratio (10-30 mL/g) on yield of celastrol from Celastrus monospermus Roxb.To optimize the conditions,we investigated the effects of parameters on the ultrasound assisted extraction (UAE) with the Box-Behnken Design (BBD),one widely used form of Response Surface Methodology (RSM).In all tested solvents,ethanol was the most effective for celastrol extraction,followed by methanol,ethanol,ethyl acetate,n-butanol and water.A second order polynomial model was fitted well to the extraction experimental data with R2 of 0.9928.Extraction yield of 3.116 mg/g was obtained for celastrol under the optimized extraction conditions of extraction time (20 min),extraction temperature (46 ℃),ultrasonic power (60 W) and S/S ratio (30 mL/g).Experimental validation was performed,and the experimental values agreed well with the predicted values.The results indicated that the UAE was good extraction material for celastrol from C.monospermus Roxb.