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【目的】观察肿瘤坏死因子-α(TNF-α)及其可能性受体(sTNF-R1和sTNF-R2)在支气管哮喘患者中的表达,探讨它们在支气管哮喘炎症机制中的作用。【方法】采用ELISA法检测哮喘急性发作期和哮喘缓解期患儿及健康对照组儿童血清TNF-α、sTNF-R1和sTNF-R2的蛋白浓度。【结果】哮喘急性发作期组血清TNF-α、sTNF-R1和sT-NF-R2的蛋白浓度[分别为(3.19±1.68)(、4.69±2.30)、(14.32±6.19)]ng/mL显著高于对照组[(1.96±0.86)、(2.07±0.86)、(4.75±1.68)]ng/mL(P均<0.01);哮喘缓解期组sTNF-R1和sTNF-R2的蛋白浓度[分别为(2.59±1.21)、(11.57±4.78)]ng/mL显著低于哮喘急性发作期组(分别为P<0.01和<0.05),而TNF-α的蛋白浓度(2.75±1.31)ng/mL与哮喘急性发作期组相比差异无统计学意义(P>0.05);哮喘缓解期组TNF-α和sTNF-R2的蛋白浓度显著高于对照组(P均<0.01),而sTNF-R1的蛋白浓度与对照组相比差异无统计学意义。sTNF-R1与sTNF-R2的表达水平呈显著正相关(n=99,r=0.239,P<0.05),但sTNF-R1和sTNF-R2的表达水平分别与TNF-α之间无显著相关性(P>0.05)。【结论】TNF-α、sTNFR1和sTNFR2可能参与了哮喘的炎症发病过程,哮喘患者血清TNF-α、sTNF-R1和sT-NF-R2水平增高可能被作为病情活动的指标之一。
【Objective】 To investigate the expression of tumor necrosis factor-α (TNF-α) and its possible receptors (sTNF-R1 and sTNF-R2) in patients with bronchial asthma and explore their roles in the pathogenesis of bronchial asthma. 【Methods】 Serum levels of TNF-α, sTNF-R1 and sTNF-R2 were detected by ELISA in children with acute asthma and asthma remission. 【Results】 The protein concentrations of serum TNF-α, sTNF-R1 and sT-NF-R2 in acute exacerbation of asthma group [(3.19 ± 1.68), 4.69 ± 2.30, (14.32 ± 6.19) ng / mL, (1.96 ± 0.86), (2.07 ± 0.86) and (4.75 ± 1.68) ng / mL, respectively (all P <0.01). The protein concentrations of sTNF-R1 and sTNF-R2 in remission group were (2.59 ± 1.21), (11.57 ± 4.78) ng / mL were significantly lower than those in the acute exacerbation stage of asthma (P <0.01 and <0.05, respectively) The protein levels of TNF-α and sTNF-R2 in asthmatic remission group were significantly higher than those in control group (all P <0.01), while those of sTNF-R1 protein There was no significant difference in concentration between the two groups. There was a significant positive correlation between sTNF-R1 and sTNF-R2 (n = 99, r = 0.239, P <0.05), but there was no significant correlation between sTNF-R1 and sTNF- (P> 0.05). 【Conclusion】 TNF-α, sTNFR1 and sTNFR2 may be involved in the pathogenesis of asthma. Elevated levels of serum TNF-α, sTNF-R1 and sT-NF-R2 may be used as indicators of disease activity.