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来自中国云南、辽宁、山东 3省的烟草寄生疫霉 (Phytophthoraparasiticavar.nicotianae)菌株的致病性已被划分为 3种致病类型组 ,即强致病性组、中致病性组和弱致病性组。上述省是中国的烟草主产区 ,选自云南省的 15个烟草寄生疫霉菌株、山东省的 13个烟草寄生疫霉菌株和辽宁省的 2 0个烟草寄生疫霉菌株 ,选择 3个烟草栽培品种在温室内进行接种试验测定不同菌株的致病性分化。提取受试菌株的DNA ,利用PCR技术对受试菌株的模板DNA进行随机多态性扩增分析 ,对扩增DNA片段谱带借助于UPGMA分析法构建遗传树 ,结果表明 ,受试菌株被划分为4个遗传聚类组 ,每个遗传聚类组内包括不同的烟草寄生疫霉致病性菌株 ,而且来自于不同烟区相同的致病性菌株和每种致病性的不同菌株皆不属于同一个遗传聚类组内。结果表明RAPD -PCR的遗传标记分析结果与不同致病性组的划分未有明显的区别。因此 ,随机多态性DNA图谱的相同与不同不能当作区分来自不同烟区的烟草寄生疫霉的致病性分化的分子检测的工具。
The pathogenicity of the strains of Phytophthora parasitica av.nicotianae from Yunnan, Liaoning and Shandong provinces in China has been divided into three groups of pathogenic types, namely strongly pathogenic, moderately pathogenic and weak Pathogenic group. The above-mentioned provinces are China’s major tobacco producing areas, 15 N. parasitica strains selected from Yunnan Province, 13 N. parasitica strains from Shandong Province and 20 parasitic Phytophthora infestans strains from Liaoning Province. Three tobacco plants Inoculation tests of cultivated cultivars in the greenhouse determined the pathogenic differentiation of different strains. The DNA of the tested strains was extracted, and the template DNA of the tested strains was amplified by random amplified polymorphic DNA (PCR), and the genetic DNA was amplified by UPGMA. The results showed that the tested strains were divided Is four genetic cluster groups, each genetic cluster group includes different pathogenic strains of P. parasiticus, but also from different pathogenic strains of the same tobacco and each pathogenicity of different strains are not Belong to the same genetic cluster group. The results showed that there was no significant difference between RAPD-PCR genetic marker analysis and that of different pathogenicity groups. Therefore, the same and differences in randomized polymorphic DNA profiles can not be used as a tool for molecular detection to distinguish pathogenic differentiation of Phytophthora infestans from different tobacco areas.