论文部分内容阅读
免疫PCR方法的原理是通过应用一个对DNA和抗体具有双重结合活性的连接分子,使作为标志物的DNA分子特异性地结合到抗原-抗体复合物上,形成一种特异性抗原-抗体-DNA缀合物,附着的DNA标志物可用适宜的引物进行PCR扩增,特异性PCR产物的存在证明DNA标志物分子特异性地附着于抗原-抗体复合物上,进而证明有抗原存在。在此方法中,链霉亲和素-蛋白A(SPA)嵌合蛋白被用作连接分子,链霉亲和素-SPA具有两种不同的特异结合能力,即链霉亲和素对生物素的结合力和SPA对坛IgG Fc段的结合力。这种对生物素和抗
The principle of the immunological PCR method is that a DNA molecule as a marker is specifically bound to an antigen-antibody complex by using a linker molecule that has dual binding activity to DNA and an antibody to form a specific antigen-antibody-DNA Conjugates and attached DNA markers can be amplified by PCR with suitable primers. The presence of specific PCR products proves that the DNA marker molecules are specifically attached to the antigen-antibody complex, thereby demonstrating the presence of the antigen. In this method, a streptavidin-protein A (SPA) chimeric protein is used as the linker molecule and streptavidin-SPA has two different specific binding capacities, namely streptavidin to biotin And the binding capacity of the SPA to the IgG Fc fragment of the altar. This biotin and anti-