论文部分内容阅读
目的:探讨黄连素(Berberine,BBR)在小鼠心肌梗死(myocardial infarction,MI)后心室重构中的作用,并比较BBR预处理(BBR pre-treatment,preBBR)和BBR后处理(BBR post-treatment,postBBR)给药的效果。方法:将60只C57BL/6小鼠随机分为4组,分别为假手术组、单纯MI对照组、MI+preBBR组及MI+postBBR组,每组15只。MI模型采用前降支结扎法制备。MI+preBBR组在MI模型制备前2周开始用BBR(100 mg·kg~(-1)·d~(-1))每天灌胃,持续至MI后28 d;MI+postBBR组在MI模型制备后4 h开始用BBR(100 mg·kg~(-1)·d~(-1))每天灌胃,持续至MI后28 d。记录实验期间小鼠生存情况。MI后28天采用小动物超声测定左心室收缩功能;取心脏组织,测定心脏大小和重量;ELISA方法测定血浆BNP水平;Masson染色评价心肌纤维化程度。结果:与单纯MI对照组相比,MI+preBBR组及MI+postBBR组小鼠生存率提高、心脏收缩功能增强、心脏变小、心脏重量减轻、血浆BNP水平降低、心肌纤维化明显改善。其中,MI+preBBR组上述指标的改善程度优于MI+postBBR组。结论:BBR可抑制MI后心室重构,且BBR预处理效果优于后处理。
OBJECTIVE: To investigate the effect of berberine (BBR) on ventricular remodeling after myocardial infarction (MI) in mice and compare the effects of BBR pre-treatment (preBBR) and BBR post- treatment, postBBR) administration. Methods: Sixty C57BL / 6 mice were randomly divided into 4 groups: sham operation group, MI control group, MI + preBBR group and MI + postBBR group, 15 rats in each group. MI model using anterior descending branch ligation preparation. The MI + preBBR group was orally administered with BBR (100 mg · kg -1 · d -1) 2 weeks before the MI model was administered until 28 days after MI. MI + postBBR group BBR (100 mg · kg -1 · d -1 · d -1) was given to the rats intragastrically every day for 4 hours and continued until 28 days after MI. Record the survival of mice during the experiment. The left ventricular systolic function was measured by small animal ultrasound 28 days after MI. Cardiac tissue was taken for determination of heart size and weight. Plasma BNP level was measured by ELISA. Masson staining was used to evaluate the degree of myocardial fibrosis. Results: The survival rate of MI + preBBR group and MI + postBBR group was significantly higher than that of MI control group only. The systolic and diastolic function of cardiac muscle in MI + preBBR group and MI + postBBR group was significantly lower than that of MI group. The heart weight was decreased, plasma BNP level was decreased and myocardial fibrosis was significantly improved. Among them, MI + preBBR group improved better than the MI + postBBR group. Conclusion: BBR can inhibit ventricular remodeling after MI, and BBR pretreatment is superior to post-treatment.