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目的考察汉防己碱(Tet)对白血病K562细胞生长的影响。方法采用体外细胞培养,即在37℃、5%CO2、饱和湿度的培养箱中培养,培养液为含10%热灭活NBS的完全RPMI1640细胞培养基。待K562细胞与药物作用48 h后,收集并处理细胞,结合MTT和Western blot法研究Tet对K562细胞生长的作用特点。结果 Tet在0.33～1.00μg/ml仅使K562及K562/ADM细胞的存活率降低至75%～80%,低浓度Tet(0.33μg/ml)使阿霉素(ADM)和顺铂(cDDP)对K562细胞生长的IC50增加(P<0.01),1.0μg/ml Tet则降低IC50,使ADM对K562/ADM细胞的IC50增加,cDDP的IC50无明显变化。0.33μg/ml Tet有提升2种细胞的P-gp和MRP1表达的作用(P<0.05)。Tet各浓度对LRP表达无明显影响。结论 Tet对K562及K562/ADM细胞生长抑制作用具浓度依耐性,低浓度具加速耐药的作用,其促进耐药细胞发展的机制至少与提升P-gp和MRP1表达有关。
Objective To investigate the effects of tetrandrine on the growth of leukemia K562 cells. Methods In vitro cell culture was performed in an incubator at 37 ℃, 5% CO2 and saturated humidity. The medium was complete RPMI1640 cell culture medium containing 10% heat inactivated NBS. After treated with K562 cells for 48 h, the cells were harvested and treated. The effects of Tet on the growth of K562 cells were studied by MTT and Western blot. Results Tet (0.33 ~ 1.00μg / ml) decreased the viability of K562 and K562 / ADM cells to 75% ~ 80% The IC50 of K562 cells increased (P <0.01). The IC50 decreased with 1.0μg / ml Tet, while the IC50 of ADM increased to K562 / ADM cells. The IC50 of cDDP did not change significantly. 0.33μg / ml Tet enhanced the expression of P-gp and MRP1 in both cell lines (P <0.05). Tet concentration had no significant effect on LRP expression. CONCLUSION: Tet can inhibit the growth of K562 and K562 / ADM cells in a concentration-dependent and drug-resistant manner at a low concentration. Tet’s mechanism of promoting the development of drug-resistant cells is at least related to enhancing the expression of P-gp and MRP1.