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目的 调查研究兰州地区婴幼儿腹泻轮状病毒的分子流行病学特点。方法 分别用酶联免疫吸附试验 (ELISA)、聚丙烯酰胺凝胶电泳 (PAGE)、逆转录 聚合酶链反应 (RT PCR)三种方法对2 0 0 0年 1～ 2月、10～ 12月 ,2 0 0 1年 1～ 3月在兰州医学院第一附属医院儿科门诊及住院部收集的 114份婴幼儿腹泻粪便标本进行轮状病毒病原检测 ,并用核酸杂交方法对轮状病毒阳性标本进行分型。结果 三种方法检测轮状病毒感染的阳性率分别为 6 1% (ELISA)、5 8% (PAGE)、5 8 8% (RT PCR) ,轮状病毒核酸的电泳型有长、短两种类别 ,以长型为主 (81 8% ) ,偶有长、短型混合感染。流行的G型主要为G3(6 1% ) ,其次为G2 (2 5 4 % )、G1(13 6 % ) ,未见G4型 ,8例未能分型。P型主要为P1A(77 4 % ) ,其次为P1B(2 2 6 % ) ,无P2型。结论 婴幼儿轮状病毒腹泻在兰州地区的流行病学特点与世界各国报道的情况相仿 ,但 2 0 0 0年 1～ 2月、10～ 12月 ,2 0 0 1年 3月期间 ,其流行的主要基因型为G3型。本次研究结果从分子水平初步揭示了兰州地区轮状病毒的流行情况 ,为轮状病毒疫苗在该地区的应用提供了流行病学资料
Objective To investigate the molecular epidemiology of infantile diarrhea rotavirus in Lanzhou area. Methods Three methods, enzyme-linked immunosorbent assay (ELISA), polyacrylamide gel electrophoresis (PAGE) and reverse transcription-polymerase chain reaction (RT PCR), were performed on January-February 2000, October-December , From January to March 2001, 114 cases of diarrhea stool samples collected from pediatric outpatient and inpatient department of the First Affiliated Hospital of Lanzhou Medical College were tested for rotavirus pathogen, and rotavirus positive samples were detected by nucleic acid hybridization Type. Results The positive rates of rotavirus infection by the three methods were 61% (ELISA), 58% (PAGE) and 588% (RT PCR) respectively. There were two types of rotavirus nucleic acid electrophoresis, Type, mainly long (81 8%), occasionally long, short mixed infection. The prevalence of G type was mainly G3 (61%), followed by G2 (245%) and G1 (13.6%), with no G4 type and 8 cases failed to type. P-type mainly P1A (77 4%), followed by P1B (2 2 6%), no P2 type. Conclusions The epidemiological characteristics of infants and young children with rotavirus diarrhea in Lanzhou are similar to those reported in other countries in the world. However, prevalence of diarrhea in infants and young children in Lanzhou from January to February in 2000, October to December and March 2001 The main genotype is G3. The results of this study initially revealed the prevalence of rotavirus in Lanzhou from the molecular level and provided epidemiological data for the application of rotavirus vaccine in the area