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目的:建立护肝抗原胶囊的质量控制方法。方法:采用HPLC法测定护肝抗原胶囊中黄芩素和五味子醇甲含量,同时建立胶囊中女贞子、刺五加、五味子、虎杖、柴胡、丹皮、黄芩、桑寄生TLC定性鉴别。结果:HPLC法测出黄芩素、五味子醇甲分别在0.124~1.246、0.226~2.26μg范围内,有良好的线性关系,线性方程分别是:Y=2701.98 X+11.42(r=0.9999)和Y=1711.31 X+12.32(r=0.9999);加样回收率分别是98.02%(RSD=2.54%)和98.51%(RSD=1.37%)。同时,薄层色谱中检出了刺五加、五味子、女贞子、虎杖、刺五加、柴胡、丹皮、黄芩、桑寄生的特征斑点。结论:该方法简单可行,能用于控制护肝抗原胶囊的质量。
Objective: To establish a quality control method for liver protection antigen capsule. Methods: HPLC method was used to determine baicalein and schisandrin in Hugan Zhi Capsules. TLC was also used to identify Fructus Ligustri Lucidi, Acanthopanax, Schisandra, Polygonum cuspidatum, Radix Bupleuri, Radix Bupleuri, Results: There was a good linear relationship between baicalein and schisandrin in the range of 0.124-1.246 and 0.226-2.26 μg, respectively. The linear equations were: Y = 2701.98 X + 11.42 (r = 0.9999) and Y = 1711.31 X + 12.32 (r = 0.9999). The recoveries were 98.02% (RSD = 2.54%) and 98.51% (RSD = 1.37%) respectively. At the same time, the spots of Acanthopanax senticosus, Schisandra chinensis, Ligustrum lucidum, Polygonum cuspidatum, Acanthopanax senticosus, Radix Bupleuri, Radix Paeoniae Rubra, Radix Scutellariae and Sanguinea were detected by TLC. Conclusion: This method is simple and feasible and can be used to control the quality of liver protection antigen capsule.