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目的探讨实时荧光定量PCR(RQ-PCR)检测孕妇外周血中胎儿游离DNA对预测及预防妊娠期高血压疾病的应用价值。方法选15例子痫前期孕妇及正常孕妇20例,用RQ-PCR法检测各例血浆中GAPDH及SRY水平,通过2-ΔΔct法分析两组孕妇间的差异。结果22例孕男胎检出SRY21例,13例孕女胎均未检出,子痫前期组胎儿DNA水平明显高于正常组(P=0.009),两者比值为3.57。结论RQ-PCR法检测孕妇外周血中胎儿DNA可作为预测及预防妊高征的一种有效手段。
Objective To investigate the value of real-time fluorescent quantitative PCR (RQ-PCR) in the detection of fetal DNA in pregnant women’s peripheral blood to predict and prevent gestational hypertension. Methods Twenty patients with preeclampsia and 20 normal pregnant women were enrolled in this study. The plasma levels of GAPDH and SRY were measured by RQ-PCR, and the differences between the two groups were analyzed by 2-ΔΔct method. Results Twenty-two cases of SRM were detected in 21 cases of SRY and none of the 13 cases were detected. The fetal DNA level in preeclampsia group was significantly higher than that in normal group (P = 0.009), with a ratio of 3.57. Conclusion The detection of fetal DNA in peripheral blood of pregnant women by RQ-PCR can be used as an effective means to predict and prevent PIH.