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近年来越来越多的证据表明,结节病时各种类型的肺损伤是吞噬细胞产生的高活性氧基作用的结果。后者可引起急性肺血管损伤,促使大量多核白细胞(PMN)和肺泡巨噬细胞(PAM)在肺组织内聚集。本研究采用3-氨基苯二酰肼(Luminol)增强细胞的化学发光(CL),以检测外周血 PMN 和肺灌洗液中 PAM 在吞噬酵母多糖和乳胶微粒时所产生的荧光反应。用闪烁计数器检测 CL。共测正常对照者14例,结节病患者16例(其中6例接受强的松治疗)。支气管肺泡细胞(BAC)是从收集的支气管肺泡灌洗液而得;周围血白细胞是用低分子右旋糖酐沉淀法分
There is growing evidence in recent years that various types of lung injury during sarcoidosis are the result of the high reactive oxygen species produced by phagocytes. The latter can cause acute pulmonary vascular injury, prompting a large number of multicellular leukocytes (PMN) and alveolar macrophages (PAM) in lung tissue aggregation. In this study, chemiluminescence (CL) of cells was enhanced by 3-aminophthalide (Luminol) to detect the fluorescence of PAM in peripheral blood PMN and lung lavage when phagocytosis of zymosan and latex particles. Detects CL with scintillation counter. A total of 14 cases of normal controls were tested, and 16 cases of sarcoidosis (6 cases were treated with prednisone). Bronchoalveolar cells (BACs) are derived from bronchoalveolar lavage fluid collected; peripheral blood leucocytes are fractionated by low molecular weight dextran precipitation