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从质粒ｐＵＣ19得到无内含子的完全的前β乳球蛋白,并插入到质粒ｐＡＭ91中以产生质粒ｐＭＪＡＢＬＧ。其中前β乳球蛋白基因处于磷酸甘油酸激酶上游和下游侧翼序列的调控下。用ｐＭＡＢＬＧ转化酿酒酵母ＪＲＹ188、ＢＪ5464和ＡＨ22。菌株ＡＨ22(18ｍｇ/Ｌ)表达水平最好。在所
Intron-free complete pre-beta lactoglobulin was obtained from plasmid pUC19 and inserted into plasmid pAM91 to generate plasmid pMJABLG. Wherein the pre-beta lactoglobulin gene is under the control of phosphoglycerate kinase upstream and downstream flanking sequences. S. cerevisiae JRY188, BJ5464 and AH22 were transformed with pMABLG. Strain AH22 (18mg / L) the best expression level. In the place