论文部分内容阅读
目的:检测卵巢上皮性癌组织中磷酸化信号转导及转录活化因子3(p-STAT3)和Ki67蛋白的表达。方法:应用免疫组织化学SP法检测35例卵巢上皮性癌组织(高分化11例,中分化12例,低分化12例;Ⅰ、Ⅱ期16例,Ⅲ、Ⅳ期19例;有淋巴结转移20例,无淋巴结转移15例)、20例良性卵巢上皮性肿瘤组织及30例正常卵巢组织中p-STAT3和Ki67蛋白的表达。结果:正常卵巢组织、良性卵巢上皮性肿瘤组织及卵巢上皮性癌组织中p-STAT3的表达水平分别为(43.20±3.18),(50.12±4.36)及(92.57±5.26),3者相比,差异有统计学意义(F=7.605,P=0.001);Ki67的表达水平分别为(7.69±1.21)、(12.53±2.62)及(16.18±5.32),3者相比,差异有统计学意义(F=3.925,P=0.029)。卵巢上皮性癌组织中p-STAT3和Ki67的表达与临床分期、分化程度及淋巴结转移有关(p-STAT3:t/F=6.921、3.865和7.763,P均<0.05;Ki67:t/F=6.917、3.870和4.021,P均<0.05)。卵巢上皮性癌组织中p-STAT3与Ki67的表达呈正相关(rS=0.563,P<0.001)。结论:卵巢上皮性癌组织中p-STAT3与Ki67的表达升高,联合检测2者对判断卵巢上皮性癌的恶性程度和预后有一定价值。
Objective: To detect the expression of phospho-signal transducer and activator of transcription 3 (p-STAT3) and Ki67 in epithelial ovarian cancer. Methods: Immunohistochemical SP method was used to detect 35 cases of ovarian epithelial carcinoma (11 cases were well differentiated, 12 cases were moderately differentiated, 12 cases were poorly differentiated; 16 cases were stage Ⅰ and Ⅱ, 19 cases were stage Ⅲ and Ⅳ; Cases without lymph node metastasis in 15 cases), 20 cases of benign ovarian epithelial tumor tissue and 30 cases of normal ovarian tissue p-STAT3 and Ki67 protein expression. Results: The expression of p-STAT3 in normal ovarian tissue, benign ovarian epithelial tumor and epithelial ovarian cancer were (43.20 ± 3.18), (50.12 ± 4.36) and (92.57 ± 5.26), respectively The difference was statistically significant (F = 7.605, P = 0.001); Ki67 expression levels were (7.69 ± 1.21), (12.53 ± 2.62) and (16.18 ± 5.32), respectively, the difference was statistically significant F = 3.925, P = 0.029). The expression of p-STAT3 and Ki67 in epithelial ovarian cancer was correlated with clinical stage, differentiation degree and lymph node metastasis (p-STAT3: t / F = 6.921,3.865 and 7.763, P <0.05, Ki67: t / F = 6.917 , 3.870 and 4.021, P <0.05). There was a positive correlation between the expression of p-STAT3 and Ki67 in epithelial ovarian cancer (rS = 0.563, P <0.001). Conclusion: The expression of p-STAT3 and Ki67 in epithelial ovarian cancer is increased, and the combined detection of them is of value in judging the malignancy and prognosis of epithelial ovarian cancer.