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[Objective] The aim was to screen out the differentiation medium formula for the virus-free test-tube seedling from stem tips of Dioscorea oppasita.[Method] With MS as the basic medium,a three-factor and three-level test was designed with 6-benzyl aminopurine (6-BA),2,4-dichlorophenoxyacetic acid (2,4-D) and naphthylacetic acid (NAA) as treatment factors and different hormones concentrations as levels.The screening of the differentiation medium for virus-free seedling from the stem tips of D.oppasita was studied.[Result] Formula of MS+2mg/L 6-BA+1mg/L 2,4-D+ 3mg/L NAA was the optimum differentiation medium for the virus-free seedling of D.oppasita.[Conclusion] D.oppasita cultured in the screened medium could reach a favorable balance between dedifferentiation and differentiation of stem tips.The medium is a suitable differentiation medium for culturing the virus-free seedlings of D.oppasita stem tips.
[Objective] The aim was to screen out the differentiation medium formula for the virus-free test-tube seedling from stem tips of Dioscorea oppasita. [Method] With MS as the basic medium, a three-factor and three-level test was designed with 6-benzyl aminopurine (6-BA), 2,4-dichlorophenoxyacetic acid (2,4-D) and naphthylacetic acid (NAA) as treatment factors and different hormones concentrations as levels. The screening of the differentiation medium for virus-free [Result] Formula of MS + 2 mg / L 6-BA + 1 mg / L 2,4-D + 3 mg / L NAA was the optimum differentiation medium for the virus-free seedling of D.oppasita cultured in the screened medium could reach a favorable balance between dedifferentiation and differentiation of stem tips. The medium is a suitable differentiation medium for culturing the virus-free seedlings of D.oppasita stem tips.