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本研究对2015年收集的春玉米区玉米穗腐病样本的病原菌进行了分离鉴定。结果表明,禾谷镰孢复合种(F.graminearum species complex)的总分离频率最高,为35.90%,为优势菌。进一步分析显示,山西、河北、吉林和黑龙江省以禾谷镰孢复合种为主,分离频率分别为81.25%,75.00%,44.00%和44.44%;内蒙古以拟轮枝镰孢(F.verticillioides)为主,分离频率为56.25%;辽宁省拟轮枝镰孢和哈茨木霉(Trichoderma harzianum)的分离频率分别为34.48%和31.03%;而在陕西省,禾谷镰孢复合种、拟轮枝镰孢和亚粘团镰孢(F.subglutinans)分离频率均为28.57%。以禾谷镰孢复合种的EF-1α基因序列为基础构建系统发生树,进一步对分离到的禾谷镰孢复合种进行亚种鉴定。结果表明,春玉米区禾谷镰孢复合种为禾谷镰孢和布氏镰孢,且以布氏镰孢为主。
In this study, the pathogen of corn ear rot samples collected in 2015 was isolated and identified. The results showed that F. graminearum complex (F. graminearum species complex) the total separation frequency of 35.90%, the dominant bacteria. Further analysis showed that Fusarium graminearum species were the most common in Shanxi, Hebei, Jilin and Heilongjiang Provinces with the frequencies of 81.25%, 75.00%, 44.00% and 44.44%, respectively. In Inner Mongolia, F. verticillioides , With the frequency of 56.25%. The isolation frequency of Fusarium verticillata and Trichoderma harzianum in Liaoning Province were 34.48% and 31.03%, respectively. In Shaanxi Province, Fusarium graminearum complex, Fusarium and Fusobacterium (F.subglutinans) separation frequency was 28.57%. The phylogenetic tree was constructed based on the sequence of EF-1α gene of Fusarium graminearum and the subspecies of Fusarium graminearum isolates were further identified. The results showed that the Fusarium graminearum isolates in spring corn were Fusarium graminearum and Fusarium graminearum, and Fusarium graminearum was the dominant species.