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本文比较了用ELISA法与解剖蚊体法检测恶性疟子孢子的效果。以实验室喂养的乌头按蚊叮咬带配子体的志愿者。每组20只蚊,于第10,11及12天以氯伤致死,解剖10只蚊,检查唾腺中的子孢子;另10只置小指管中冷冻后保存于有干燥剂的容器内,置-20℃ 2～3周。每只蚊加50μl含NP-40的BB液(含1％ BSA,0.5％酪蛋白,0.03％硫汞撤及0.01％酚红的PBS)研磨成匀浆后,再加350μl BB液,即成蚊提取液。保存于-20℃备用。以Writz改良的ELISA二点法进行检
This article compared the ELISA method and dissection mosquito method to detect the effect of P. falciparum spores. Anopheles aconite bites the volunteers with gametophytes in laboratory. 20 mosquitoes in each group were killed by chlorine injury on the 10th, 11th and 12th days. Ten mosquitoes were dissected and the sporozoites in the salivary glands were examined. The other 10 were stored in desiccated containers after being frozen, Set -20 ℃ 2 to 3 weeks. Each mosquito plus 50μl of BB solution containing NP-40 (PBS containing 1% BSA, 0.5% casein, 0.03% thiomersal with 0.01% phenol red) was homogenized and then 350 μl of BB solution was added Mosquito extract. Store at -20 ° C until use. Writz modified ELISA two-point method for testing