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以加拿大不列颠哥伦比亚省甲虫致死黑松为原料,通过SO2催化蒸汽爆破方式对其进行预处理,并对预处理后的原料进行酶水解和乙醇发酵研究,以考察预处理方式结合酶水解对黑松制取乙醇的影响。结果表明,较低的SO2吸收率和原料含水率(干基)影响了预处理效果;水洗对预处理后原料的酶水解效率没有显著的影响;当水解底物质量浓度由20mg/mL增加到150mg/mL时,水解率均在42%左右,底物质量浓度的增加纤维素的水解率没有受到显著影响。从发酵结果看,黑松经汽爆预处理后,不进行水洗处理更有利于后续发酵。分步糖化发酵乙醇得率可以达最大乙醇得率的66%,而同步糖化水乙醇得率为55%。由此可见,低浓度SO2催化汽爆预处理低湿度黑松不能得到较好的预处理效果,需进一步优化。
In order to study the pretreatment process of enzymatic hydrolysis and ethanol fermentation, the black pine Preparation of ethanol effects. The results showed that the lower SO2 absorption rate and the moisture content of the raw material (dry basis) affected the pretreatment effect. The washing efficiency had no significant effect on the enzymatic hydrolysis efficiency of the pretreated material. When the mass concentration of hydrolysis substrate was increased from 20mg / mL At 150mg / mL, the hydrolysis rate was about 42%. The increase of substrate concentration did not affect the hydrolysis rate of cellulose significantly. From the fermentation results, the black pine by steam explosion pretreatment, without washing treatment is more conducive to follow-up fermentation. Stepwise saccharification and fermentation of ethanol yields up to 66% of the maximum ethanol yield, while the simultaneous saccharification of ethanol yields 55%. It can be seen that low concentration SO2 catalytic steam explosion pretreatment of low humidity black pine can not get a better pretreatment effect, need to be further optimized.