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目的观察并比较不同比例的七叶皂苷钠样品的抗炎及毒性作用,筛选确定纯化分离七叶皂苷钠的最佳活性部位,为优化精制工艺提供参考.方法采用耳肿胀及腹腔毛细血管通透性2种炎症模型,观察四个具有七叶皂苷钠A、B、C、D不同比例样品的抗炎作用,并测定静脉给药的LD50.结果所研究的七叶皂苷钠四个样品腹腔注射给药能明显抑制二甲苯所致小鼠耳肿胀,皮下注射给药均能明显抑制醋酸所致小鼠腹腔毛细血管通透性的增加.四个样品的LD50分别为9811199.76mg·kg-1、9811123.43mg·kg-1、9811164.14mg·kg-1、9803159.57mg·kg-1.结论四个样品的抗炎作用以981119、981112和981116略好,毒性以981119和980315较低,因此样品981119所含七叶皂苷钠为最佳活性部位.
Objective To observe and compare the anti-inflammatory and toxic effects of different proportions of sodium aescinate, to screen and determine the best active site for the purification and separation of sodium aescinate, and to provide reference for the optimization of the refining process. The method uses ear swelling and peritoneal capillary permeability Two models of inflammation were used to observe the anti-inflammatory effects of four samples with different ratios of sodium aescinate A, B, C, and D, and to determine the LD50 of intravenous administration. Results Four samples of sodium aescinate studied were injected intraperitoneally Administration can significantly inhibit xylene-induced ear swelling in mice, and subcutaneous injections significantly inhibited the increase in acetic acid-induced permeability of the capillary in the abdominal cavity of the mice. The LD50 of the four samples was 981119.76 mg·kg-1, respectively. 9811123.43mg·kg-1, 9811161.14mg·kg-1, 9803095.57mg·kg-1. Conclusion The anti-inflammatory effects of the four samples are slightly better with 981119, 981112 and 981116, and the toxicity is lower with 981119 and 980315, therefore the sample 981119 Containing sodium aescinate as the best active site.