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目的:研究内皮素1(ET-1)在肺癌组织中的表达与肺癌分型及分级的关系。方法:采用免疫组织化学ABC染色及图像分析技术测定ET-1阳性率及含量。结果:104例肺癌组织ET-1阳性表达主要分布在胞浆内。腺癌、鳞癌和大细胞肺癌ET-1表达较高,表达阳性率分别为71.4%(20/28)、57.1%(16/68)及40.0%(8/20)。小细胞肺癌表达最低,表达阳性率为21.4%(6/28)。腺癌、鳞癌阳性表达的图像分析表明,肿瘤分化程度越低ET-1含量越高,肿瘤分化程度越高ET-1含量越低。结论:ET-1普遍存在于肺癌细胞中,腺癌和鳞癌高表达,ET-1含量可能与肿瘤分化程度有一定关系。ET-1有望作为肺腺癌、鳞癌的一个检测指标。
Objective: To investigate the relationship between the expression of endothelin-1 (ET-1) in lung cancer and the classification and grading of lung cancer. Methods: Immunohistochemical ABC staining and image analysis were used to determine the positive rate and content of ET-1. Results: The positive expression of ET-1 in 104 cases of lung cancer mainly distributed in cytoplasm. The positive rates of ET-1 in adenocarcinoma, squamous cell carcinoma and large cell lung cancer were 71.4% (20/28), 57.1% (16/68) and 40.0% (8/20), respectively. Small cell lung cancer expression was the lowest, the positive rate was 21.4% (6/28). The image analysis of positive expression of adenocarcinoma and squamous cell carcinoma showed that the lower the degree of tumor differentiation is, the higher the content of ET-1 is, and the higher the degree of tumor differentiation is, the lower the content of ET-1 is. CONCLUSION: ET-1 is commonly found in lung cancer cells, with high expression of adenocarcinoma and squamous cell carcinoma. ET-1 level may be related to the degree of tumor differentiation. ET-1 is expected as a lung adenocarcinoma, a detection of squamous cell carcinoma.