论文部分内容阅读
目的观察SA4503对抑郁大鼠海马环磷酸腺苷应答元件结合蛋白(CREB)及脑源性神经营养因子(BDNF)的影响。方法雄性Wistar大鼠32只随机均分为对照组(C组)和三个剂量SA4503组(K1-3组)。实验药物干预前1d,大鼠强迫游泳15min建立抑郁大鼠模型。药物干预当日,分别腹腔注射1ml等容量的生理盐水(C组)、SA4503 2.5mg/kg(S1组)、5mg/kg(S2组)、10mg/kg(S3组)。给药30min后将大鼠再次行强迫游泳实验5min,记录其不动时间。随后取海马组织测定CREB及BDNF含量。结果与C组相比,S1-3组呈剂量依赖性地减少大鼠强迫游泳不动时间及增加海马CREB和BDNF表达(P<0.05)。结论 SA4503对大鼠的抗抑郁作用可能与前额皮层CREB及BDNF表达上调有关。
Objective To investigate the effects of SA4503 on CREB and BDNF in hippocampus of depressed rats. Methods 32 male Wistar rats were randomly divided into control group (C group) and three doses of SA4503 group (K1-3 group). One day before the intervention of experimental drugs, rats were forced to swim for 15 minutes to establish a depressive rat model. On the day of drug intervention, intraperitoneal injection of 1 ml of normal saline (group C), SA4503 2.5 mg / kg (group S1), 5 mg / kg (group S2) and 10 mg / kg (group S3) respectively. 30min after administration, the rats were forced to swim for 5min again, and the immobility time was recorded. Subsequently, hippocampal tissue was taken to measure the content of CREB and BDNF. Results Compared with group C, group S1-3 decreased the forced swimming time and the expression of CREB and BDNF in hippocampus in a dose - dependent manner (P <0.05). Conclusion The antidepressant effect of SA4503 on rats may be related to the upregulation of CREB and BDNF in the prefrontal cortex.