论文部分内容阅读
为了寻找一种简便、有效测定桑树细菌性枯萎病病菌(Eenterobacter cloacae)致病性的方法,通过离体注射菌液(注射法)、离体牙签蘸菌苔刺伤(牙签法)、离体泡菌液、苗期菌液灌根和苗期泡菌液共5种人工接种方法测定病原菌对桑树的致病性。结果表明,5种人工接种方法仅有注射法和牙签法能成功将病原菌接种至桑树嫩梢,其中牙签法试验组桑树嫩梢的发病率和病情指数最高,分别达到78.99%和54.17,与注射法相比较达到差异极显著水平。因此,简便、有效的牙签法可以作为室内测定桑树细菌性枯萎病病菌致病性及桑树抗细菌性枯萎病育种材料鉴定的首选人工接种病原菌的方法。
In order to find out a simple and effective method to determine the pathogenicity of the bacterial pathogen Eenterobacter cloacae, in vitro injection of bacterial liquid (injection method) Five kinds of artificial inoculation methods were used to determine the pathogenicity of pathogen to mulberry. The results showed that inoculation method and toothpick method could successfully inoculate pathogen into the shoots of mulberry only by five methods of inoculation, in which the incidence and the disease index of mulberry shoots in toothpick test group were the highest (78.99% and 54.17 respectively) France reached a very significant difference compared to the level. Therefore, the simple and effective method of toothpick can be used as the method of artificial inoculation of the pathogen for pathogen determination of mulberry bacterial wilt pathogen and the identification of mulberry bacterial wilt disease breeding material.