论文部分内容阅读
目的:研究明日叶查尔酮对2型糖尿病大鼠骨骼肌胰岛素抵抗的干预作用。方法:将2型糖尿病大鼠随机分成四组,高、中、低剂量组分别每日经口灌胃给予明日叶查尔酮30、10和5mg/(kg.bw),糖尿病对照组给予等量生理盐水。各组均以高脂饲料喂养。四周后采用葡萄糖氧化酶法检测空腹血糖;放射免疫法检测血清胰岛素含量;免疫组化法检测葡萄糖转运体1和葡萄糖转运体4蛋白表达水平。结果:经图像分析,高剂量组骨骼肌细胞中葡萄糖转运体1和葡萄糖转运体4蛋白表达平均光密度值分别为0.054±0.0064和0.063±0.0139,均较糖尿病对照组显著性升高(P<0.05)。高剂量组空腹血糖和胰岛素水平分别为(12.3±1.64)mmol/L和(25.65±3.34)(μIU/mL),均较糖尿病对照组显著性降低(P<0.05)。结论:明日叶查尔酮可增加2型糖尿病大鼠骨骼肌葡萄糖转运体1和葡萄糖转运体4蛋白表达水平,降低空腹血糖和胰岛素水平,改善胰岛素抵抗状况。
OBJECTIVE: To study the intervention effect of etrilone on insulin resistance in skeletal muscle of type 2 diabetic rats by Tomorrow. Methods: Type 2 diabetic rats were randomly divided into four groups. The high, middle and low dose groups were orally administered with 30, 10 and 5 mg / (kg · bw) Normal saline. All groups were fed with high fat diet. Glucose oxidase method was used to detect fasting blood glucose after four weeks. Serum insulin level was measured by radioimmunoassay. Expressions of glucose transporter 1 and glucose transporter 4 protein were detected by immunohistochemistry. Results: The average optical density of glucose transporter 1 and glucose transporter 4 in high dose group were 0.054 ± 0.0064 and 0.063 ± 0.0139, respectively, which were significantly higher than those in diabetic control group (P < 0.05). Fasting blood glucose and insulin levels were (12.3 ± 1.64) mmol / L and (25.65 ± 3.34) (μIU / mL) in high dose group, which were significantly lower than those in diabetic control group (P <0.05). Conclusion: Yechal ketones can increase the protein expression of glucose transporter 1 and glucose transporter 4 in skeletal muscle of type 2 diabetic rats, reduce fasting blood glucose and insulin levels, and improve insulin resistance status.