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研究了低氮营养和水杨酸对离体黄瓜子叶开花的影响。结果表明，低氮营养（总氮量为３０．０ｍｍｏｌ·Ｌ－１，ＮＯ－３Ｎ∶ＮＨ＋４Ｎ为２∶１）显著促进子叶花芽分化；低浓度水杨酸（ＳＡ１０．０μｍｏｌ·Ｌ－１）对花芽分化也有明显促进作用，高浓度ＳＡ（高于１０．０μｍｏｌ·Ｌ－１）则起抑制作用；低氮营养条件下施以低浓度ＳＡ（１０．０μｍｏｌ·Ｌ－１）对于促进子叶花芽分化效果最佳。另外，硝酸还原酶（ＮＲ）和苯丙氨酸解氨酶（ＰＡＬ）的活性和花芽分化有紧密关系。ＮＲ活性与花芽分化状态呈负相关，而ＰＡＬ活性则和花芽分化状态呈正相关。ＮＲ与ＰＡＬ活性分别在培养后第１０天与第２０天达到最高。而过氧化氢酶（ＣＡＴ）的活性则在诱导后呈下降趋势，未发现其与子叶的花芽分化有明显关系。
The effects of low N nutrition and salicylic acid on the flowering of in vitro cucumber cotyledons were studied. The results showed that low nitrogen nutrition (30.0mmol·L-1 total nitrogen, NO-3N: NH4N was 2:1) significantly promoted cotyledon floral bud differentiation; low concentrations of salicylic acid (SA10.0μmol·L -1) significantly inhibited flower bud differentiation. High concentration of SA (10.0μmol·L-1) inhibited the growth of seedlings. Low concentration of SA (10.0μmol·L-1) Promote cotyledon flower bud differentiation best. In addition, nitrate reductase (NR) and phenylalanine ammonia lyase (PAL) activity and flower bud differentiation are closely related. NR activity was negatively correlated with flower bud differentiation status, while PAL activity was positively correlated with flower bud differentiation status. The activities of NR and PAL reached the highest on the 10th day and the 20th day respectively. However, the activity of catalase (CAT) showed a downward trend after induction, and it was not found that catalase had a significant relationship with flower bud differentiation of cotyledons.