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研究了在PBS缓冲介质中,一种检测癌胚抗原的新型免标记免疫电化学传感器的制备,将石墨烯、二茂铁的高效催化及壳聚糖的优良生物相容性和成膜性、离子液体的导电性等优势充分结合构建了电化学免疫传感器。通过循环伏安法及交流阻抗对修饰的电极进行表征,在最优条件下,癌胚抗原的质量浓度在0.2～50.0 ng/mL的范围内与差分脉冲伏安法峰电流呈良好的线性关系,回归方程为Δi=0.38-1.31ρ,相关系数分别为0.9967,检测限为0.06 ng/mL,该传感器可用于人血清样品的测定。
A novel immunochromatographic sensor for the detection of carcinoembryonic antigen was developed in PBS buffer medium. The high-efficiency biocatalysis of graphene and ferrocene and the excellent biocompatibility and film-forming property of chitosan, Ionic liquids and other advantages of the full integration of the electrochemical immunosensor constructed. The modified electrodes were characterized by cyclic voltammetry and AC impedance. Under optimal conditions, the mass concentration of carcinoembryonic antigen showed a good linear relationship with the peak current of differential pulse voltammetry in the range of 0.2-50.0 ng / mL , The regression equation was Δi = 0.38-1.31ρ, the correlation coefficient was 0.9967, the detection limit was 0.06 ng / mL, the sensor can be used for the determination of human serum samples.