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目的鉴别血尿来源,提高出血部位的诊断率。方法利用ABC免疫染色法观察了148例血尿标本中红细胞表面THP(Tamm-HorsfalProtein)含量,同时与红细胞形态学法进行了对比。结果肾小球血尿(69例)红细胞THP染色阳性率大于70%,非肾小球性血尿(79例)阳性率小于30%。THP免疫染色正确率为95.2%,形态学检验的正确率为89.9%,二者总符合率为92.5%。结论THP抗体ABC免疫染色法能较客观的鉴别血尿来源,提高血尿出血部位的诊断率
Purpose To identify the source of hematuria and improve the diagnosis of bleeding site. Methods The content of THP (Tamm-HorsfalProtein) on erythrocytes in 148 cases of hematuria was observed by ABC immunostaining, and compared with erythrocyte morphometry. Results The positive rate of erythrocyte THP staining was more than 70% in glomerular hematuria (69 cases) and less than 30% in non - glomerular hematuria (79 cases). The correct rate of THP immunostaining was 95.2%, the accuracy of morphological test was 89.9%, the total coincidence rate was 92.5%. Conclusion THP antibody ABC immunostaining method can more objectively identify the source of hematuria, improve the diagnosis of hematuria bleeding site