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为了解缺锌是否导致体液及细胞免疫功能失调,诱发口腔溃疡的发生，采用吡啶偶氮萘酚比色法测定了血清中的锌，用免疫荧光法计数外用血T淋巴细胞亚群，用免疫比浊法测定血清免疫球蛋白。通过各50例实验观察表明，口腔溃疡组血清锌偏低，且T淋巴细胞亚群比值失调，与健康对照组比较，有显著性差异。
To understand whether zinc deficiency caused humoral and cellular immune dysfunction and induce the occurrence of oral ulcer, Zinc in serum was determined by pyridine-azo-naphthol colorimetric assay, and T-lymphocyte subsets of external blood were counted by immunofluorescence method. Turbidimetric determination of serum immunoglobulin. Through the 50 cases of experimental observations show that the oral ulcer group serum zinc is low, and T lymphocyte subsets imbalance, compared with the healthy control group, there was a significant difference.