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背景:绞股蓝对实验性脑缺血大鼠具有脑保护功能,血管性痴呆大鼠同样具有海马神经元的缺血缺氧性损伤,绞股蓝对此是否也有保护作用?目的:观察绞股蓝总皂苷(gypenosides,GP)对血管性痴呆(vascularde-mentia,VD)大鼠海马神经元型一氧化氮合酶(neuronalnitricoxidesyn-thase,nNOS)阳性神经元及核酸的保护作用。设计:随机对照研究。地点和对象:研究在武警医学院中心实验室进行,二级雄性wistar大鼠30只,体质量240～260g,由天津市医学实验动物中心提供。干预:采用随机数字法将30只雄性大鼠分为对照组、模型组及给药组。模型组用改进的Pulsinelli4-血管阻断方法建立大鼠VD模型。给药组:灌胃给药GP200mg/kg,按照大鼠VD模型的制备方法进行手术。对照组:同样进行手术但不灼烧颈动脉,不夹闭颈总动脉。主要观察指标:①大鼠海马nNOS表达。②大鼠海马DNA和RNA荧光染色强度。结果:模型组大鼠海马CA1区和CA3区nNOS阳性神经元数分别为(20.47±4.22)个和(25.47±3.52)个,明显少于对照组犤(24.73±5.72)和(37.13±5.10)个犦(P<0.05),DNA和RNA吖啶橙染色后的荧光强度(反映DNA和RNA含量)也明显减弱。给药组海马CA1区和CA3区nNOS阳性神经元数分别为(30.00±3.63)个和(38.00±5.00)个,比模型组明显增多(P<0.001);给药组海马DNA和
BACKGROUND: Gynostemma has protective effect on rats with experimental cerebral ischemia. Vascular dementia rats also have hypoxic-ischemic injury of hippocampal neurons. Does Gynostemma also have a protective effect? Objective: To observe the gypenosides (gypenosides) , GP) The protective effect of neuronal nitric oxide synthase (nNOS)-positive neurons and nucleic acid in hippocampus of vascular dementia (VD) rats. Design: Randomized controlled study. Location and Object: The study was conducted at the Central Laboratory of the Armed Police Medical School. Twenty male Wistar rats, weighing 240 to 260 g, were provided by Tianjin Medical Experimental Animal Center. Intervention: Thirty male rats were divided into control group, model group and administration group using random number method. The model group established a rat VD model using a modified Pulsinelli 4-vessel occlusion method. In the administration group, GP 200 mg/kg was intragastrically administered, and surgery was performed according to the preparation method of rat VD model. Control group: The same surgery was performed but the carotid artery was not burned and the common carotid artery was not clipped. MAIN OUTCOME MEASURES: 1 Expression of nNOS in hippocampus of rats. 2 Rat hippocampal DNA and RNA fluorescence staining intensity. Results: The number of nNOS positive neurons in hippocampal CA1 and CA3 was (20.47±4.22) and (25.47±3.52) in model group, which were significantly lower than those in control group (24.73±5.72) and (37.13±5.10). One sputum (P<0.05), the fluorescence intensity of DNA and RNA stained with acridine orange (reflecting DNA and RNA content) was also significantly reduced. The number of nNOS-positive neurons in the hippocampal CA1 and CA3 regions of the treated group was (30.00±3.63) and (38.00±5.00), respectively, which was significantly higher than that of the model group (P<0.001).