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目的探讨雾化吸入氟碳化合物(PFC)对急性肺损伤大鼠肺表面活性物质、肺表面活性物质相关蛋白-A(SP-A)与肿瘤坏死因子α(TNF-α)的影响。方法A组采用腹腔注射脂多糖(LPS)诱导大鼠急性肺损伤模型,并雾化吸入PFC进行干预;B组以生理盐水代替LPS作为对照。观察PFC对鼠动脉血气、支气管肺泡灌洗液(BALF)中总蛋白、双饱和磷脂酰胆碱(DPPC)、SP-A、TNF-α及动物死亡率的影响。结果与B组相比,A组PFC吸入明显提高动脉血氧分压(PaO2),增加BALF中SP-A及DPPC的浓度,降低BALF中总蛋白与TNF-α浓度,降低24 h内的死亡率(P<0.01)。结论PFC雾化吸入可通过增加SP-A及DPPC浓度、降低BALF中总蛋白与TNF-α浓度降低急性肺损伤大鼠死亡率。
Objective To investigate the effects of inhalation of fluorocarbon (PFC) on pulmonary surfactant, pulmonary surfactant protein-A (SP-A) and tumor necrosis factor-α (TNF-α) in rats with acute lung injury. Methods Group A was induced by intraperitoneal injection of lipopolysaccharide (LPS) to induce acute lung injury in rats, and inhaled PFC was used for intervention. Group B received physiological saline instead of LPS as control. The effects of PFC on total arterial blood gas, total protein in bronchoalveolar lavage fluid (BALF), dual-saturated phosphatidylcholine (DPPC), SP-A, TNF-α and animal mortality were observed. Results Compared with group B, PFC inhalation in group A significantly increased PaO2, increased the concentration of SP-A and DPPC in BALF, decreased the concentration of total protein and TNF-α in BALF and decreased the death within 24 h Rate (P <0.01). Conclusions PFC inhalation can reduce the death rate of acute lung injury in rats by increasing the concentration of SP-A and DPPC, decreasing the total protein and TNF-α in BALF.