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目的:为了研究尼可地尔(nicorandil,Nic)抗心律失常的作用机制.方法:体外培养乳鼠心肌细胞并观察Nic对培养心肌细胞自发性搏动频率及动作电位的影响.结果:Nic能使体外培养乳鼠心肌细胞自发性搏动频率下降.Nic 60 μmol L~(-1)对CaCl_21 mmol L~(-1)及异丙肾上腺素2 μmol L~(-1)增加的细胞搏动率有抑制作用.Nic 32和160 μmol L~(-1)能使动作电位幅度(APA)及最大上升速率(V_(max))明显下降,使动作电位时程(APD_(50),APD_(90))缩短,搏动周期(SCL)显著延长.结论:Nic的抗心律失常机制与其对心肌细胞的直接作用有关.
Objective: To study the mechanism of antiarrhythmic effect of nicorandil (Nic) in neonatal rat cardiomyocytes.Methods: The cardiomyocytes were cultured in vitro and the effect of nicotine on the spontaneous beat frequency and action potential of cultured cardiomyocytes was observed.Results: Nic The frequency of spontaneous pulsation of neonatal rat cardiomyocytes decreased in vitro.Nic 60 μmol L -1 inhibited the cell beating rate of CaCl_ (21 mmol L -1) and isoproterenol (2 μmol L -1) (APD_ (50), APD_ (90)) were significantly decreased with the addition of Nic 32 and 160 μmol L -1, which could significantly decrease the APA and the maximal rate of increase (V max) Shortened, and prolonged period of beating (SCL) significantly.Conclusion: Nicotinic antiarrhythmic mechanism is related to its direct effect on cardiomyocytes.