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目的:观察复方金黄Ⅰ号及组成该方的各单味药体外对流感病毒(A1,A3)的抑制作用,比较复方与单味药体外抗病毒效果,为临床合理用药提供依据。方法:建立体外病毒感染模型,采用细胞病变法(CPE)和MTT法观察金黄Ⅰ号及单味药在不同时间给药对流感病毒感染的抑制作用。结果:对A1,A3流感病毒,金黄Ⅰ号在治疗、预防以及感染病毒同时加药3种条件下细胞存活量较病毒对照组均有显著提高,抑制率高于部分单味药和利巴韦林对照组;除金银花、浮小麦在治疗条件下对A3流感病毒的抑制作用不明显外,其余各单味药在3种条件下对2种病毒均有显著抑制作用。结论:金黄Ⅰ号具有体外抗流感病毒作用,总体而言其抗病毒作用优于组成该方的各单味药。
OBJECTIVE: To observe the inhibitory effect of Fufang JINYUAN I and its single herbs on influenza virus (A1 and A3) in vitro and to compare the antiviral effects of compound and single herbs in vitro and to provide basis for clinical rational drug use. Methods: The model of virus infection in vitro was established. The inhibition of influenza virus infection by different doses of Golden I and single herb was observed by cytopathic effect (CPE) and MTT assay. Results: The cell survival rates of A1, A3 and Golden Ⅰ were significantly higher than those of the virus control group in the three conditions of treatment, prevention and virus infection, the inhibitory rate was higher than that of some single drug and ribavirin In the control group, the inhibitory effects of honeysuckle and floating wheat on the A3 influenza virus were not obvious under the treatment conditions, while the rest of the single herbs showed significant inhibitory effect on the two viruses under the three conditions. Conclusion: Golden I has the effect of anti-influenza virus in vitro, in general its anti-virus effect is better than the composition of the side of the single drug.