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目的研究海南眼树莲醇提取物的急性毒性和抗炎活性部位。方法用最大浓度、最大给药体积的乙醇提取物灌胃给药,观察急性毒性;溶剂萃取法制备不同极性部位萃取物,采用二甲苯致小鼠耳肿胀模型、醋酸致小鼠腹腔毛细血管通透性模型、大鼠足跖肿胀增加模型筛选抗炎活性部位。结果海南眼树莲小鼠最大耐受生药量为206.45g/kg。乙酸乙酯萃取物和正丁醇萃取物能明显抑制二甲苯引起的小鼠耳肿胀、醋酸引起的小鼠毛细血管通透性增加、鸡蛋清诱导大鼠足肿胀。结论海南眼树莲乙醇提取物无明显毒性,其乙酸乙酯萃取物和正丁醇萃取物为眼树莲的抗炎活性部位。此实验为进一步进行该植物抗炎活性成分的提取分离提供了实验基础。
Aim To study the acute toxicity and anti-inflammatory activity of the extract of Eimeria in Hainan. Methods The ethanol extract with maximum concentration and maximum administration volume was intragastrically administrated to observe the acute toxicity. Solvent extraction was used to prepare the extract with different polarity sites. Xylene-induced mouse ear swelling model, acetic acid induced peritoneal capillary Permeability model, rat paw swelling model to screen anti-inflammatory active sites. The results showed that the maximum tolerated crude drug dose was 206.45g / kg in P. erecta mouse. Ethyl acetate extract and n-butanol extract can significantly inhibit xylene-induced mouse ear swelling, acetic acid-induced increased capillary permeability in mice, egg white induced foot swelling in rats. Conclusion There is no obvious toxicity in Ethanol extract of Eupolyphaga nivalis, and its ethyl acetate extract and n-butanol extract are the anti-inflammatory active sites of Eimeria. This experiment provides the experimental basis for the further extraction and separation of the anti-inflammatory active ingredients of the plant.