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目的:建立T细胞免疫功能重建模型,研究免疫与胰腺癌淋巴转移的关系,探讨免疫的影响因素。方法 :建立裸鼠胰腺癌淋巴转移模型及T细胞免疫功能重建的模型。将裸鼠分为免疫功能重建组(R1)和免疫功能未重建组(R2)。比较两组成瘤时间差异。病理切片观察淋巴结转移程度。流式细胞仪检测两组裸鼠外周血及脾脏CD3+CD4+、CD3+CD8+细胞百分比。结果 :R1组成瘤时间(8.5±0.6)d,R2组成瘤时间(6.4±0.8)d,两者差异具有统计学意义(P<0.05)。R1组胰腺癌淋巴转移受到一定程度的抑制,髂总动脉旁淋巴结的转移率明显低于R2组(P<0.05)。R1组裸鼠外周血以及脾脏CD3+CD4+、CD3+CD8+细胞百分比明显高于R2组。结论:T细胞免疫功能重建裸鼠模型较好地模拟免疫环境,为胰腺癌淋巴转移的免疫相关研究提供动物实验平台。
OBJECTIVE: To establish a model of T cell immune function reconstruction and to study the relationship between immunity and lymph node metastasis of pancreatic cancer and to explore the influencing factors of immunity. Methods: To establish a model of lymphatic metastasis of pancreatic cancer in nude mice and reconstruction of T cell immune function. Nude mice were divided into immune functional reconstruction group (R1) and immune functional non-reconstruction group (R2). Comparison of tumor formation time difference between the two groups. Pathological observation of lymph node metastasis. The percentage of CD3 + CD4 +, CD3 + CD8 + cells in peripheral blood and spleen of two groups were detected by flow cytometry. Results: The time of tumor formation in group R1 was (8.5 ± 0.6) d and that in group R2 (6.4 ± 0.8) d, the difference was statistically significant (P <0.05). The lymphatic metastasis of pancreatic cancer in group R1 was inhibited to a certain degree, and the metastasis rate of lymph node in common iliac artery was significantly lower than that in group R2 (P <0.05). The percentage of CD3 + CD4 +, CD3 + CD8 + cells in peripheral blood and spleen of R1 group was significantly higher than that of R2 group. CONCLUSION: T cell immune function reconstruction nude mouse model can better simulate the immune environment and provide animal experimental platform for immune related research of lymphatic metastasis of pancreatic cancer.