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应用双温聚合酶链反应（ＰＣＲ）检测喉乳头瘤石蜡包埋组织中的人乳头瘤病毒（ＨＰＶ）ＤＮＡ。结果与常规ＰＣＲ（三温循环）相同：３０例喉乳头瘤中ＨＰＶ总检出率为４６．７％（１４／３０），ＨＰＶ－６／１１／１６的检出率分别为４０％（１２／３０）、１６．７％（５／３０）和６．７％（２／３０），ＨＰＶ－６的检出率极显著高于ＨＰＶ－１１／６（ｘ２＝１０．６，Ｐ＜０．０１）。初步结果表明，本地区喉乳头瘤主要与ＨＰＶ－６的感染密切相关。该法具有缩短检测时间、节省一个恒温水浴箱等优点。
Human papillomavirus (HPV) DNA in paraffin-embedded tissues of laryngeal papilla was detected by dual-temperature polymerase chain reaction (PCR). The results were the same as those of the conventional PCR (three-temperature cycle): the total HPV positive rate was 46.7% (14/30) in 30 cases of laryngeal papilloma and 40% (12%) of HPV-6/11 / /30), 6.7% (5/30) and 6.7% (2/30) respectively. The detection rate of HPV-6 was significantly higher than that of HPV-11/6 .01). Preliminary results show that the region mainly laryngeal papilloma HPV-6 infection are closely related. The method has the advantages of shortening detection time, saving a constant temperature water bath and so on.