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目的制备高纯度的银杏原花青素,为系统研究银杏来源的原花青素提供物质基础。方法利用NP-HPLC方法指导大孔树脂及聚酰胺树脂的纯化工艺,分析并定量所得原花青素提取物中黄酮杂质的组成及含量,用DPPH法比较银杏和葡萄籽来源的原花青素的抗氧化活性。结果制备得到的银杏原花青素提取物中原花青素的含量>90%,黄酮杂质含量仅为3.44%,DPPH清除率IC50为22.87μg·mL~(-1),葡萄籽原花青素IC50为36.99μg·mL~(-1)。结论本研究工艺可制备获得高纯度的银杏原花青素,其抗氧化活性较葡萄籽原花青素更强。
Objective To prepare high purity ginkgo proanthocyanidins and provide the material basis for the systematic study of proanthocyanidins derived from ginkgo. Methods The purification process of macroporous resin and polyamide resin was guided by NP-HPLC method. The composition and content of flavonoids in the obtained proanthocyanidin extract were analyzed and quantified. The antioxidant activity of proanthocyanidins derived from Ginkgo biloba and grape seed were compared by DPPH method. Results The proanthocyandin content of extract was> 90%, the content of flavonoid was only 3.44%, the IC50 of DPPH was 22.87μg · mL -1, the progesterone of grape seed was 36.99μg · mL ~ (-1) -1). Conclusion The study can be prepared to obtain high purity ginkgo proanthocyanidins, the antioxidant activity of grape seed proanthocyanidins stronger.