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目的研究大鼠染锰后对其子代新生鼠脑组织热应激蛋白合成的影响。方法雌性大鼠随机分为４组，腹腔注射氯化锰两周，隔日一次，剂量分别为０、７５、１５、３０ｍｇ／ｋｇ；受孕后以同样方法继续染锰一周。分娩后对其新生鼠脑组织用ｗｅｓｔｅｒｎ斑点印迹法检测热应激蛋白７０，并测定超氧化物歧化酶（ＳＯＤ）。结果与对照组相比，ＳＯＤ活性在各组间差异无显著意义（Ｐ＞００５）；热应激蛋白７０，３０ｍｇ／ｋｇ组明显高于对照组（Ｐ＜００１），７５和１５ｍｇ／ｋｇ组略有增加，但差异无显著意义（Ｐ＞００５）。结论亲代高剂量染锰可诱导子代新生鼠脑组织热应激蛋白７０合成增高，但这种效应在锰的胚胎毒性中的作用和机理仍有待进一步阐明。
Objective To study the effects of manganese on the synthesis of heat stress protein in brain tissue of offspring neonatal rats. Methods Female rats were randomly divided into 4 groups, intraperitoneal injection of manganese chloride for two weeks, once every other day, the doses were 0,7 5,15,30 mg / kg; in the same way after conception, continue to dye manganese for one week. After birth, heat shock protein 70 was detected by western blot in brain tissue of newborn rats, and superoxide dismutase (SOD) was measured. Results Compared with the control group, there was no significant difference in the activity of SOD between the groups (P> 0.05); the levels of heat stress protein 70 and 30 mg / kg were significantly higher than those in the control group (P <001) 5 and 15mg / kg group increased slightly, but no significant difference (P> 005). Conclusion The high dose of Mn2 + could induce the synthesis of heat stress protein 70 in the brain of neonatal rats. However, the role and mechanism of this effect in the embryotoxicity of manganese remains to be elucidated.