论文部分内容阅读
目的:探讨人乳头瘤病毒(HPV)16早期编码蛋白E6、细胞周期素D1(cyclin D1)及人端粒酶逆转录酶(human telom-erase transcriptase,hTERT)在鼻咽癌发生发展中的作用及临床意义。方法:采用免疫组织化学SP法检测HPV16E6、cyclinD1、hTERT在鼻咽癌组织和鼻咽部慢性炎症组织中的表达情况,分析其与鼻咽癌临床病理参数间的关系及其对预后的影响。结果:HPV16E6、cyclin D1、hTERT在鼻咽癌组织中阳性表达率分别为62.5%(35/56)、50.0%(28/56)、67.9%(38/56),明显高于鼻咽部慢性炎症组(P<0.05)。三者阳性表达率随组织学分级的降低、临床分期的增加及淋巴结转移而升高(P<0.05),与患者年龄及性别无关(P>0.05)。HPV16E6分别与cyclin D1和hTERT呈正相关(r=0.480,P<0.001;r=0.494,P<0.001)。HPV16E6、cyclin D1和hTERT阳性患者平均生存期、中位生存期均低于阴性患者(P<0.05)。结论:HPV16E6、cyc-lin D1、hTERT的高表达可能与鼻咽癌的发生及发展相关。HPV16E6与cyclin D1,HPV16E6与hTERT之间可能存在相互作用;同时检测这3个指标可能对判断鼻咽癌患者预后有指导意义。
AIM: To investigate the role of early coding protein E6, cyclin D1 and hTERT in the development of nasopharyngeal carcinoma (HPV) 16 in human papillomavirus (HPV) 16 And clinical significance. Methods: Immunohistochemical SP method was used to detect the expression of HPV16E6, cyclinD1, hTERT in nasopharyngeal carcinoma and nasopharyngeal tissues. The relationship between HPV16E6, cyclinD1, hTERT and the clinicopathological parameters of nasopharyngeal carcinoma and its prognostic significance were analyzed. Results: The positive rates of HPV16E6, cyclin D1 and hTERT in nasopharyngeal carcinoma were 62.5% (35/56), 50.0% (28/56) and 67.9% (38/56), respectively, which were significantly higher than those of chronic nasopharyngeal Inflammation group (P <0.05). The positive expression rate of the three with histological grade decreased, the clinical stage increased lymph node metastasis and increased (P lt; 0.05), and the age and gender of the patients (P gt; 0.05). HPV16E6 was positively correlated with cyclin D1 and hTERT (r = 0.480, P <0.001; r = 0.494, P <0.001). The mean survival and median survival of HPV16E6, cyclin D1 and hTERT positive patients were lower than those of negative patients (P <0.05). Conclusion: The high expression of HPV16E6, cyc-lin D1 and hTERT may be related to the occurrence and development of nasopharyngeal carcinoma. HPV16E6 and cyclin D1, HPV16E6 and hTERT may exist interaction; simultaneous detection of these three indicators may be of prognostic significance for the judgment of patients with nasopharyngeal carcinoma.