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目的:探讨TGF-β2反义寡核苷酸对兔角膜基质成纤维细胞转化、增殖的作用,揭示其对角膜创伤修复的影响。方法:新西兰大白兔28只,双眼均制备角膜基质创伤模型,右眼为实验组,用浸有TGF-β2反义寡核苷酸的8-0薇乔缝线缝合角膜创口;左眼为对照组,用普通8-0薇乔缝线缝合角膜创口,分别于4,7,14,21d处死动物,取角膜后行免疫组化(α-SMA和PCNA)染色和电镜观察。结果:术后各时间段均发现,实验组α-SMA和PCNA阳性的成纤维细胞数明显少于对照组,但成纤维细胞的超微结构实验组和对照组比较无明显区别。结论:TGF-β2反义寡核苷酸可抑制兔角膜基质成纤维细胞转化、增殖,为调控角膜基质伤口修复提供了一个新的途径。
Objective: To investigate the effect of TGF-β2 antisense oligonucleotide on the transformation and proliferation of rabbit corneal stromal fibroblasts, and to reveal its effect on corneal wound repair. Methods: Corneal stromal trauma model was established in 28 eyes of New Zealand white rabbits. The right eye was treated by corneal wound with 8-0 Vicat sutured with TGF-β2 antisense oligonucleotide. The left eye was control Group, with ordinary 8-0 Vicat suture stitched corneal wounds, animals were sacrificed at 4,7,14,21d, corneal immunohistochemical (α-SMA and PCNA) staining and electron microscopy. Results: The number of α-SMA and PCNA-positive fibroblasts in the experimental group was significantly less than that in the control group at each time period. However, there was no significant difference between the experimental group and the control group in the ultrastructure of fibroblasts. CONCLUSION: TGF-β2 antisense oligonucleotide can inhibit the transformation and proliferation of rabbit corneal stromal fibroblasts, which provides a new approach for the regulation of corneal wound repair.