论文部分内容阅读
目的:制备包载雷公藤甲素的壳聚糖纳米粒系统(triptolide-chitosan nanoparticles,TP-CS NPs),并考察其理化性质及体外释药性能。方法:采用非溶剂辅助络合-化学交联法制备壳聚糖纳米粒。利用透射电镜、粒度仪、HPLC法等对制备的纳米粒性质进行表征。结果:制得的纳米粒呈球形,平均粒径为(151.2±5.4)nm,Zeta电位(20.1±1.2)m V,纳米粒收率(78.0±3.3)%,包封率(77.0±1.2)%,载药量(2.0±0.4)%,体外模拟释药结果表明载药纳米粒药物释放速率在24 h内持续稳定。结论:非溶剂辅助络合-化学交联法制备的雷公藤甲素壳聚糖纳米粒简便可靠,体外释药具有明显的缓释作用。
OBJECTIVE: To prepare triptolide-chitosan nanoparticles (TP-CS NPs) containing triptolide-chitosan nanoparticles and study its physicochemical properties and in vitro drug release properties. Methods: Chitosan nanoparticles were prepared by non-solvent assisted complexation-chemical crosslinking method. The prepared nanoparticles were characterized by transmission electron microscopy, particle size analyzer and HPLC. Results: The average diameter of the nanoparticles was (151.2 ± 5.4) nm, the Zeta potential was (20.1 ± 1.2) mV, the yield of nanoparticles was (78.0 ± 3.3)% and the encapsulation efficiency was (77.0 ± 1.2) %, Drug loading (2.0 ± 0.4)%, in vitro drug release results showed that the release rate of drug-loaded nanoparticles sustained within 24 h. Conclusion: Triptolide-chitosan nanoparticles prepared by non-solvent assisted complexation-chemical crosslinking method are simple and reliable, and have a sustained release effect in vitro.