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目的 :观察虎仗提取物白藜芦醇对胃癌细胞HGC2 7细胞周期和增殖的影响及凋亡诱导作用 .方法 :采用MTT法和软琼脂集落形成实验观察白藜芦醇对细胞增殖的影响 ,Annxin V&PI染色及流式细胞仪检测观察白藜芦醇对细胞周期的影响及诱导凋亡的作用 .结果 :MTT实验发现白藜芦醇作用HGC2 7细胞 2 4h后 ,细胞存活率显著下降 (P <0 .0 1)并呈剂量依赖性 ,软琼脂集落形成实验发现HGC2 7细胞在0 .5mmol/L白藜芦醇作用下无细胞集落的形成 ,Annxin V&PI染色及流式细胞仪检测发现白藜芦醇可引起HGC2 7阻滞于G1期 ,并可诱导HGC2 7发生凋亡 (8.39% ) .结论 :白藜芦醇可抑制胃癌细胞HGC2 7增殖并可诱导细胞发生凋亡 .
Objective: To observe the effect of resveratrol on the cell cycle and proliferation of human gastric cancer cell line HGC277 and apoptosis induction. Methods: To observe the effect of resveratrol on cell proliferation by MTT method and soft agar colony formation assay. Annxin V&PI staining and flow cytometry were used to observe the effect of resveratrol on the cell cycle and the induction of apoptosis. Results: MTT assay showed that the viability of cells was significantly decreased after resveratrol treated HGC27 cells for 24 hours (P<0.05). <0. 0 1) and in a dose-dependent manner, soft agar colony formation experiments revealed that HGC27 cells did not develop colony formation with 0.5 mmol/L resveratrol, and were stained with Annexin V&PI staining and flow cytometry. Resveratrol can cause HGC2 7 block in G1 phase, and can induce apoptosis of HGC27 (8.39%). Conclusion: Resveratrol can inhibit the proliferation of gastric cancer cells HGC2 7 and induce apoptosis.