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目的:采用逆流萃取的方法,在两种不同的萃取流速下,考察两种不同产地栀子的栀子苷含量及稳定性,以期对栀子药材的粗提工艺提供一定的参考。方法:采用HPLC法测定不同栀子逆流萃取液中栀子苷含量。以十八烷基硅烷键合硅胶为填充剂C18色谱柱(5μm,4.6×250 mm);流动相乙腈一水(15∶85);流速1.0 mL·min-1;检测波长为238 nm;柱温30℃。结果:两种不同产地不同逆流萃取条件下,栀子苷均在5 h达到最大值,种植栀子比野栀子中栀子苷含量高,且同一产地栀子在B流速时大于A流速时栀子苷含量,1个月后重复测定1次,栀子苷含量稳定。结论:HPLC简便快速、灵敏度高、重复性好、稳定性好,可为栀子提取工艺研究提供参考。
OBJECTIVE: To investigate the content and stability of geniposide in two different origins of Gardenia jasminoides by using countercurrent extraction at two different extraction rates, and to provide some references for the crude extraction of Gardenia jasminoides. Methods: The contents of geniposide in Gardenia countercurrent extract were determined by HPLC. C 18 column (5μm, 4.6 × 250 mm) with octadecylsilane bonded silica as the filler; mobile phase acetonitrile-water (15:85); flow rate 1.0 mL · min-1; detection wavelength at 238 nm; Temperature 30 ℃. Results: Under the conditions of different countercurrent extraction, the concentration of geniposide reached the maximum at 5 h, the content of geniposide in Gardenia ziyobi than those in Gardenia zizi was higher than that in the same origin, Geniposide content, 1 month after repeated determination of 1, geniposide content was stable. Conclusion: HPLC is simple, rapid, sensitive, reproducible and stable, which can provide reference for the extraction of gardenia.