论文部分内容阅读
目的:探讨结肠癌组织中癌症高表达蛋白(Hec1)的表达与肿瘤浸润、转移及预后的关系。方法:采用免疫组化SP法检测20例正常结肠组织、40例癌旁组织及癌组织中Hec1的表达,用蛋白印迹法检测上述组织中Hec1蛋白的表达。结果:在正常结肠组织、癌旁组织及癌组织中,Hec1蛋白的阳性表达率分别为0、20.0%和67.5%,各组织中Hec1蛋白的表达差异均有统计学意义,P<0.05。Hec1蛋白的表达与结肠癌的临床病理分期、侵袭转移显著相关,在发生淋巴道转移及低分化的肿瘤组织中,Hec1蛋白高表达。蛋白印迹法结果表明,在正常结肠组织、癌旁组织及癌组织中,Hec1蛋白的平均表达量的分别为0、0.13±0.02、0.87±0.15,差异有统计学意义,P<0.05。结论:Hec1蛋白的过度表达可能在结肠癌的发生和发展中起重要作用,联合应用Hec1蛋白阳性表达率与其临床病理学分级,有助于正确判断患者的预后。
Objective: To investigate the relationship between the expression of Hec1 protein and tumor invasion, metastasis and prognosis in colon cancer. Methods: The expression of Hec1 in 20 normal colon tissues, 40 adjacent tissues and cancer tissues was detected by immunohistochemical SP method. The expression of Hec1 protein was detected by Western blotting. Results: The positive rates of Hec1 protein were 0, 20.0% and 67.5% in normal colorectal tissues, paracancerous tissues and cancerous tissues respectively. There was significant difference in the expression of Hec1 protein in all tissues (P <0.05). The expression of Hec1 protein is significantly correlated with the clinicopathologic stage, invasion and metastasis of colon cancer. Hec1 protein is highly expressed in the tumor tissues with lymphatic metastasis and poorly differentiated. The results of Western blotting showed that the average expression level of Hec1 protein in normal colorectal tissues, paracancerous tissues and cancerous tissues were 0, 0.13 ± 0.02 and 0.87 ± 0.15 respectively, with statistical significance (P <0.05). Conclusion: The overexpression of Hec1 protein may play an important role in the development and progression of colon cancer. The combined application of Hec1 protein and its clinicopathological grade may help to judge the prognosis of patients.