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[目的]探讨小干扰RNA(siRNA)沉默S100P基因后对胰腺癌SW1990细胞株生长的影响。[方法]化学合成针对S100P基因的siRNA,用脂质体转染试剂将siRNA体外转染至人胰腺癌SW1990细胞中,48h后收集细胞,分别提取细胞总RNA和蛋白。用实时定量RT-PCR测定S100P基因mRNA水平的表达;免疫印迹测定S100P蛋白的表达;MTT法测定细胞的生长曲线。[结果]转染siRNA后,S100P基因在mRNA水平上表达减少了77%,蛋白表达降低了74%,均显著性低于对照组。siRNA-S100P明显抑制细胞生长。[结论]应用RNAi技术沉默S100P基因可以降低人胰腺癌SW1990细胞株中S100P表达,抑制细胞生长。
[Objective] To investigate the effect of silencing S100P gene by small interfering RNA (siRNA) on the growth of pancreatic cancer cell line SW1990. [Method] siRNA targeting S100P gene was chemically synthesized. The siRNA was transfected into human pancreatic cancer cell line SW1990 in vitro using lipofectamine transfection reagent. After 48 hours, the cells were harvested, and total RNA and protein were extracted respectively. The expression of S100P mRNA was detected by real-time quantitative RT-PCR. The expression of S100P protein was detected by immunoblotting and the growth curve was measured by MTT assay. [Results] The expression of S100P gene was decreased by 77% and the protein expression was reduced by 74% after transfection with siRNA, both of which were significantly lower than those of the control group. siRNA-S100P significantly inhibited cell growth. [Conclusion] The silencing of S100P gene by RNAi can reduce the expression of S100P in human pancreatic cancer cell line SW1990 and inhibit the cell growth.