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目的对Ｙａｍ１Ｂ细胞自发产生的一种可抑制人Ｔ、Ｂ细胞增殖的可溶性因子（Ｙａｍ－ＳＦ）进行研究。方法３Ｈ－ＴｄＲ掺入法进行淋巴细胞增殖抑制测定，ＥＬＩＳＡ法测定免疫球蛋白，间接免疫荧光法测定ＩＬ－２Ｒ，ＣＴＬＬ－２细胞３Ｈ－ＴｄＲ掺入法测定ＩＬ－２活性。结果Ｙａｍ－１Ｂ培养上清（Ｙａｍ－ＳＦ）经ｐｒｏｎａｓｅ水解消化而失活，经５６℃处理１０分钟，或在ｐＨ１０环境中处理６小时抑制活性丧失，在ｐＨ２．０环境中可保持其抑制活性。凝胶过滤试验表明，培养上清抑制活性分布在分子量４３０００～６７０００范围内，且ＴＧＦ－β抗体不能阻断其抑制活性。结论Ｙａｍ－ＳＦ可能是一种新的免疫调节因子，该因子可抑制人Ｔ细胞及Ｂ细胞的增殖，并抑制正常Ｂ细胞免疫球蛋白的产生，但对ＰＨＡ剌激的人淋巴细胞ＩＬ－２的产生以及ＩＬ－２受体（ＩＬ－２Ｒ）的表达无抑制作用。
Objective To study a spontaneously soluble factor (Yam-SF) that inhibits the proliferation of human T and B cells in Yam1B cells. Methods 3H-TdR incorporation method was used to determine the inhibition of lymphocyte proliferation. Immunoglobulin was detected by ELISA. IL-2 activity was measured by 3H-TdR incorporation assay of IL-2R and CTLL-2 cells by indirect immunofluorescence. Results The Yam-SF culture supernatant (Yam-SF) was inactivated by pronase hydrolysis and digestion, and its inhibitory activity was lost after it was treated at 56 ℃ for 10 minutes or at pH10 for 6 hours, and its inhibitory activity was maintained at pH2.0 . Gel filtration tests showed that the inhibitory activity of the culture supernatant was distributed in the range of molecular weight between 43000 and 67000, and the TGF-β antibody could not block its inhibitory activity. Conclusion Yam-SF may be a new immunoregulatory factor that inhibits the proliferation of human T cells and B cells and inhibits the production of normal B cell immunoglobulins. However, it is not sensitive to PHA-stimulated human lymphocytes IL-2 Production and IL-2 receptor (IL-2R) expression.