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大豆胞囊线虫(Soybean Cyst Nematode,SCN)是一种极具破坏性的大豆寄生虫,一般可造成大豆减产10%~20%。利用植物介导RNAi是培育抗虫转基因植物新品种的有效手段之一。本研究从大豆胞囊线虫中获得了编码参与线虫m RNA代谢的关键基因-核糖体蛋白基因Hg-rps-23。采用农杆菌介导法将Hg-rps-23 RNA干扰片段导入Williams82中。PCR和Southern杂交检测结果表明,外源RNAi片段已导入受体大豆品种。病土接种鉴定结果表明,转基因大豆对大豆胞囊线虫3号生理小种抗性水平显著提高,表明利用植物介导RNAi可以有效提高大豆抗胞囊线虫水平。本研究为大豆抗胞囊线虫新材料的创制及新品种培育提供了一条新的途径。
Soybean Cyst Nematode (SCN) is a highly destructive soybean parasite that generally results in a 10% to 20% reduction in soybean yield. Plant-mediated RNAi is one of the most effective ways to cultivate new varieties of insect-resistant transgenic plants. In this study, we obtained the gene Hg-rps-23, a key gene involved in the m RNA metabolism of C. elegans, from soybean cyst nematode. Hg-rps-23 RNA interference fragment was introduced into Williams82 by Agrobacterium-mediated method. PCR and Southern blot results showed that the exogenous RNAi fragment was introduced into the recipient soybean varieties. Inoculation results showed that the transgenic soybean significantly increased the resistance to soybean cyst nematode No. 3, indicating that plant-mediated RNAi can effectively increase the level of resistance to soybean cyst nematode. This study provided a new way for the creation of new materials for soybean cyst nematode and the cultivation of new varieties.