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[目的]探讨对氨基水杨酸钠(PAS-Na)对亚慢性染锰大鼠基底前脑胆碱能神经细胞的影响。[方法]48只雄性SD大鼠被随机分为对照Ⅰ、Ⅱ组,染锰Ⅰ、Ⅱ组,PAS-Na预防(预防)组,PAS-Na治疗(治疗)组,每组8只。染锰组、预防组、治疗组大鼠每日腹腔注射氯化锰(MnCl2.4H2O 15mg/kg),每周5d,对照组腹腔注射等容量生理盐水,预防组在染锰同时每日背部皮下注射PAS-Na(200mg/kg),连续12周。13周开始,治疗组每日背部皮下注射PAS-Na(200mg/kg),同时染锰Ⅱ组、对照Ⅱ组背部皮下注射等容量生理盐水至18周。采用水迷宫实验检测大鼠学习记忆能力,免疫组化染色观察胆碱乙酰转移酶(ChAT)阳性神经元形态及数量,检测基底前脑ChAT蛋白活性。[结果]12周后,染锰组逃避潜伏期、游泳路程均比对照Ⅰ组长,预防组逃避潜伏期、游泳路程均较染锰组短(P<0.05)。染锰组基底前脑垂直支臂核(vDB)/水平支臂核(hDB)ChAT阳性细胞数较对照Ⅰ组少,预防组vDB/hDB ChAT阳性细胞数较染锰组多(P<0.05);染锰组大鼠基底前脑组织ChAT蛋白活性较对照Ⅰ组低,预防组较之回升(P<0.05)。18周后,染锰组逃避潜伏期、游泳路程都比对照组长,治疗组逃避潜伏期、游泳路程比染锰组短(P<0.05)。染锰组vDB/hDB ChAT阳性细胞数较对照组少,治疗组vDB ChAT阳性细胞数较染锰Ⅱ组多(P<0.05)。[结论]PAS-Na对亚慢性染锰引起大鼠基底前脑ChAT阳性细胞减少、ChAT蛋白活性降低和学习记忆障碍可能有拮抗作用。
[Objective] To investigate the effect of sodium aminosalicylate (PAS-Na) on basal forebrain cholinergic cells in rats with subchronic manganese exposure. [Methods] Forty eight male Sprague-Dawley rats were randomly divided into control group Ⅰ, Ⅱ, Mn Ⅰ, Ⅱ group, PAS-Na prevention group and PAS-Na treatment group. The rats in the manganese-treated group, the prophylaxis group and the treatment group were intraperitoneally injected with manganese chloride (MnCl2.4H2O 15 mg / kg) 5 days a week, and the control group were intraperitoneally injected with the same volume of normal saline. PAS-Na (200 mg / kg) was injected for 12 weeks. The rats in the treatment group were injected subcutaneously with PAS-Na (200mg / kg) on the back, and the rats in the control group were injected with the same volume of normal saline for 18 weeks. The water maze test was used to detect the learning and memory abilities of rats. The morphology and number of ChAT positive neurons were observed by immunohistochemical staining and the activity of ChAT protein in basal forebrain was detected. [Result] After 12 weeks, the escape latency and swimming distance of Mn-Mn-Mn-Zn group were all longer than that of control group Ⅰ, and the escape latency and swimming distance of prevention group were shorter than that of Mn-Mn group (P <0.05). Compared with the control group, the number of ChAT positive cells in the vDB / hDB basal forebrain in the manganese-treated group was less than that in the control group (P <0.05) The activity of ChAT protein in basal forebrain of rats in Mn-Zn group was lower than that in control group Ⅰ, and the level of ChAT in prevention group was higher than that in control group (P <0.05). Eighteen weeks later, the Mn-Mn group had longer escape latency and swimming distance than the control group. The treatment group had shorter escape latency and shorter swimming distance (P <0.05) than the Mn-Mn group. Compared with the control group, the number of vDB / hDB ChAT positive cells in the manganese-treated group was less than that in the control group, and the number of vDB ChAT positive cells in the treated group was more than that in the manganese-exposed group (P <0.05). [Conclusion] PAS-Na may be antagonistic to subchronic manganese exposure in rats with reduced basal forebrain ChAT positive cells, decreased activity of ChAT protein and learning and memory impairment.