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目的 观察慢性乙型肝炎 (CH B)患者血清、肝组织中HBVDNA含量与庚型肝炎病毒(HGV)感染的关系 ,探讨HGV感染对CH B患者乙型肝炎病毒 (HBV)复制的影响。方法 应用逆转录 聚合酶链反应 (RT PCR)、免疫组织化学法、荧光定量PCR(FQ PCR)技术方法对 5 6份CH B患者血清HGVRNA、肝组织HGVAg、血清及肝组织中HBVDNA含量分别进行了检测 ,并将血清HGVRNA与肝组织HGVAg的表达、HGVRNA、HGVAg阳性与阴性患者HBVDNA含量分别进行了对比研究。结果 血清HGVRNA、肝组织HGVAg阳性分别为 10份 (17.9% )、8份 (14 .3% )。血清HGVRNA阳性与肝组织HGVAg表达显著相关 (P <0 .0 1) ,但部分肝组织HGVAg阴性患者亦有血清HGVRNA表达。血清HGVRNA、肝组织HGVAg阳性与阴性患者血清及肝组织中HBVDNA含量差异无显著性 (P >0 .0 5 )。结论 HGV感染对CH B患者HBV复制无影响。HGV可在肝脏中复制 ,但致病性可能较微弱。
Objective To investigate the relationship between HBVDNA content and hepatitis G virus (HGV) infection in patients with chronic hepatitis B (CHB) and to investigate the effect of HGV infection on hepatitis B virus (HBV) replication in CHB patients. Methods The serum HBV DNA, HGVAg, HBV DNA in serum and liver tissues of 56 CH patients were detected by reverse transcription-polymerase chain reaction (RT PCR), immunohistochemistry and fluorescence quantitative PCR (FQ PCR) The serum HGVRNA and HGVAg expression, HGVRNA, HGVAg positive and negative HBVDNA content were compared. Results The positive rates of serum HGVRNA and HGVAg in liver tissue were 10 (17.9%) and 8 (14.3%) respectively. Serum HGVRNA positive was significantly correlated with HGVAg expression in liver tissue (P <0.01), but serum HGVRNA expression was also found in some HGVAg negative liver tissues. Serum HGVRNA, HBVDNA content in serum and liver tissue of patients with HGVAg positive and negative liver tissue was no significant difference (P> 0.05). Conclusion HGV infection has no effect on HBV replication in CHB patients. HGV can replicate in the liver, but the pathogenicity may be weak.