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目的:探讨宜昌地区亚甲蓝/光照法病毒灭活血浆临床应用的安全性和有效性。方法:随机抽取30袋新鲜冰冻血浆,检测其亚甲蓝病毒灭活前、后的总蛋白、凝血因子Ⅷ、APTT、PT、Fbg、亚甲蓝残留量;临床随机抽取400例输注亚甲蓝病毒灭活血浆的受血者,观察输注亚甲蓝病毒灭活血浆前后的体温、脉搏、呼吸、血压等生理指标。结果:未经亚甲蓝病毒灭活的新鲜冰冻血浆其总蛋白含量为(59.72±2.13)g/L、凝血因子Ⅷ(0.981 1±0.275 3)IU/ml、Fbg(2.64±0.52)g/L、APTT(13.20±0.19)s、PT(34.80±1.98)s;经亚甲蓝病毒灭活后其总蛋白含量为(57.02±3.13)g/L,回收率为95.5%、凝血因子Ⅷ(0.707 4±0.187 7)IU/ml,回收率为72.11%、Fbg(1.97±0.63)g/L,回收率为74.6%、APTT(17.3±0.27)s、PT(37.4±3.12)s、亚甲蓝残留量(0.162 7±0.102 6)mol/L;患者输注亚甲蓝病毒灭活血浆前后体温、脉搏、呼吸、血压差异无统计学意义。结论:宜昌地区亚甲蓝病毒灭活血浆的临床应用是安全有效的。
Objective: To investigate the safety and efficacy of clinical inactivation of methylene blue / light attenuated virus in Yichang area. Methods: Thirty bags of fresh frozen plasma were randomly selected to detect the total protein, coagulation factor Ⅷ, APTT, PT, Fbg and methylene blue residues before and after inactivation of methylene blue virus Blue virus inactivated blood plasma recipients, observed infusion of methylene blue virus inactivated plasma before and after the body temperature, pulse, respiration, blood pressure and other physiological indicators. Results: The total protein content of fresh frozen plasma without inactivation of methylene blue virus was (59.72 ± 2.13) g / L, that of coagulation factor Ⅷ (0.981 1 ± 0.275 3), Fbg (2.64 ± 0.52) g / L, APTT (13.20 ± 0.19) s and PT (34.80 ± 1.98) s, respectively. The total protein content after inactivation of methylene blue virus was (57.02 ± 3.13) g / L and the recovery was 95.5% 0.707 4 ± 0.187 7) IU / ml, the recoveries were 72.11%, Fbg (1.97 ± 0.63) g / L and the recoveries were 74.6%, APTT 17.3 ± 0.27 s, PT 37.4 ± 3.12 s, Blue residue (0.162 7 ± 0.102 6) mol / L; There was no significant difference in body temperature, pulse rate, respiration rate and blood pressure before and after administration of methylene blue virus inactivated plasma. Conclusion: The clinical application of methylene blue virus inactivated plasma in Yichang is safe and effective.